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12.5: Exercise 1 - Yeast transformation - Biology

The following protocol is a slight modification of the “Quick and Dirty” transformation protocol described by Amberg et al. (2005). Modifications to this method can increase its efficiency by several orders of magnitude (Gietz and Schiestl, 2007), which would be required if linear pieces of DNA were being used to tranform yeast.
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SS1_2018_Lecture_12 - Biology

Eukaryotic Gene RegulationRegulation overviewAs was previously noted, regulation is all about decision making. Gene regulation, as a general topic, is related to making decisions about the functional expression of genetic material. Whether the final product is an RNA species or a protein, the production of the final expressed product requires processes that take multiple steps.
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